Objectives: To evaluate the effects of a workplace fatigue management programme called MARIKERJA on reducing fatigue among manufacturing shift workers in Indonesia. Methods: A quasi-experimental pre- and post-test study was conducted among 116 shift workers (58 in the intervention group and 58 in the control group). The MARIKERJA programme was delivered to the intervention group for 12 weeks. Meanwhile, the control groups received the MARIKERJA intervention only at the end of the study period. Fatigue levels were measured using the Fatigue Severity Scale (FSS) in weeks 6 and 12 of the intervention. Data were analysed using a t test and a general linear model repeated measures procedure. Results: There were significant differences in fatigue scores between the control and intervention groups after the MARIKERJA intervention in week 6 (40.07 ± 8.89 vs. 27.12 ± 11.67; p < 0.001) and week 12 (38.22 ± 9.28 vs. 17.53 ± 6.54; p < 0.001). The MARIKERJA intervention effectively reduced fatigue levels by up to 37.3% (R2 =.373; p = 0.001; 95% CI = 15.82–19.25). Conclusions: The MARIKERJA intervention effectively reduces fatigue among manufacturing shift workers. © 2022, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.