Objective: Inadequate water intake can lead to various health issues, particularly dehydration in the elderly. The skin is essential in preventing dehydration, and water contributes significantly to its resilience, elasticity, and durability. This study aimed to establish a definitive relationship between fluid intake and various biophysical properties of the stratum corneum in elderly individuals. Methods: This cross-sectional study was conducted on individuals aged 65 to 80 years at the Elderly Social Institution Tresna Budi Mulia 3 in Jakarta, Indonesia, from April 2023 to August 2023. Fluid intake was assessed over 7 days. And then, the overall hydration status was evaluated by urine specific gravity, and hydration status of the stratum corneum was evaluated by skin dryness through specified symptoms sum core, skin water loss based on transepidermal water loss, and the skin's ability to retain moisture based on skin capacitance (SCap). Correlation analysis (Pearson and Spearman tests) was used to evaluate relationships between variables, and nonparametric tests (Mann-Whitney U and Friedman tests) were used to assess differences. Results: The study involved 67 subjects with a median age of 70 years. Multiple regression analysis showed that fluid intake significantly predicted urine specific gravity (coefficient = -0.01, P = 0.016), specified symptom sum score (coefficient = -9.15 × 10-5, P = 0.001), and SCap (coefficient = 0.005, P = 0.022). In addition, a weak negative correlation was found between fluid intake and overall hydration status (P < 0.001) and between fluid intake and specified symptom sum score (P < 0.001). However, no significant correlation was observed between fluid intake and transepidermal water loss (P = 0.613) or SCap (P = 0.060). Conclusion: This study highlights the potential role of fluid intake in influencing hydration status among elderly individuals. Although fluid intake is significantly associated with certain biophysical properties of the skin, it does not show a consistent correlation with all measures of skin hydration and moisture retention. © 2025 Chinese Medical Association and Hospital for Skin Diseases (Institute of Dermatology), Chinese Academy of Medical Sciences, published by Wolters Kluwer, Inc.