Left ventricular outflow tract velocity time integral (LVOT-VTI) is a noninvasive echocardiographic parameter representing stroke volume. Qur’an recitation has been reported to influence cardiovascular indicators such as heart rate variability and blood pressure, which are indirectly related to cardiac function, although its effect on direct echocardiographic parameters remains unclear. This study aimed to evaluate whether patients with heart failure with reduced ejection fraction (HFrEF) show improvement in cardiac function, as reflected by changes in LVOT-VTI, following Qur’an recitation. A case-control study was conducted involving 30 HFrEF patients divided into three groups: Group 1 recited one juz of the Qur’an daily, Group 2 recited half a juz daily, and Group 3 served as a control. Echocardiographic measurements of LVOT-VTI and ejection fraction (EF) were performed at baseline and after 30 days. Significant improvements in EF were observed in Groups 1 and 2 (p = 0.000), while no significant change was found in the control group (p = 0.260). LVOT-VTI increased significantly in Groups 1 and 2 (p = 0.020) but not in Group 3 (p = 0.090). LVOT-VTI is an independent prognostic marker in HFrEF, and these findings support further well-designed clinical trials to evaluate the clinical benefits of Qur’an recitation. © Universitas Islam Negeri Ar-Raniry.