Aims Using the technology acceptance model, this study aimed to determine whether a web-based screening tool to investigate physical burden is acceptable. Instrument & Methods This one-shot case study was conducted on 239 workers from a manufacturing company in East Java. Data were collected using a self-administered questionnaire developed through interviews, along with a 15-minute trial of a web-based workload assessment application, which was revised based on user feedback. Data were analyzed using SmartPLS software to test the technology acceptance model through path analysis. Findings Perceived utility (p<0.05) and perceived simplicity of use (p<0.05) of the application were positively and significantly influenced by Internet usage experience. Perceived usefulness had no significant effect on the intention to use the program (p>0.05), while perceived ease of use had a large and significant effect on future intention to use the application (behavioral intention; p<0.05). Additionally, actual system utilization was positively and significantly influenced by the desire to utilize the program. The data showed an excellent fit to the technology acceptance model. Conclusion The web-based screening tool to explore physical workload is well-received by workers, as its ease of use significantly influences their interest and future use. © 2025, the Authors | Publishing Rights, ASPI.