Scars that occur in surgical wounds are still a health problem worldwide. Excessive scarring significantly affects the patient's quality of life, both physically and psychologically, and can also cause pain, pruritus, and contractures and cost a considerable amount of money. Therefore, it is essential to find dressings that improve a patient's prognosis. Topical therapy for treating scars is becoming popular because it is easy to use, convenient, non-invasive, and relatively inexpensive. However, no single therapy provides optimal results to eliminate hypertrophic scar formation. For most people in the world, herbs are easier to obtain because they are widely available at relatively affordable prices, so herbal therapy is still the main choice in wound healing management. Nowadays, the selection of natural ingredients such as Royal Jelly (RJ) has increased in popularity and is in demand by the public for improving the healing of surgical wounds. The study found that the flavonoid content in the sample was equivalent to quercetin and that the total phenol content in the royal jelly extract ranged from 1.41% to 1.66%. The stability test showed that the royal jelly was stable in different conditions for up to 28 days, with no significant changes observed in pH, color, smell, texture, or spreadability. © 2023 Mendel University of Agriculture and Forestry Brno. All rights reserved.