This study investigated the expression of hepatocyte growth factor (HGF), its receptor c-MET, and the proliferation marker Ki-67 across different histological grades of ovarian carcinoma. Archived formalin-fixed, paraffin-embedded (FFPE) tissue samples (n = 32) were obtained from the IMERI-FMUI Biobank, representing confirmed ovarian cancer cases diagnosed between 2015 and 2022. Expert pathologists validated the histopathological subtypes using hematoxylin and eosin (HE) staining. Based on microscopic evaluation, the samples were classified into two groups: low-to-intermediate-grade tumors (n = 16, comprising mucinous and endometrioid subtypes) and high-grade tumors (n = 16, including clear cell and high-grade serous carcinomas). Only well-preserved samples with consistent diagnoses and sufficient tumor content were included in the study. Unstained sections of the tissue samples were subjected to immunohistochemical staining to detect the expression of HGF, c-MET, and Ki-67. Quantitative image analysis was performed using ImageJ along with the IHC Profiler plugin. To minimize bias, a blinded evaluation protocol was implemented. The staining intensity and distribution were scored semi-quantitatively, and the results were reported using H-scores and labeling indices. Statistical analyses included t-tests and Pearson correlation to compare expression levels between groups and to explore associations among the biomarkers. The study sample size was calculated to detect a minimum mean difference of 35 with a 5% alpha error and 20% beta error. This systematic approach enabled a thorough assessment of biomarker expression regarding tumor grade, supporting the potential role of HGF, c-MET, and Ki-67 as diagnostic adjuncts in ovarian carcinoma. © 2025, Editorial board of Journal of Experimental Biology and Agricultural Sciences. All rights reserved.