The oil extracted from Moringa seeds (Moringa oleifera Lam.) Moringa seeds oil (MSO) is known to have antioxidant activity potential. This study aims to obtain the optimum formulation of topical nanoemulsion preparations from Moringa seed oil, including its evaluation in stability and activity antioxidant. Moringa seed oil was characterized and then formulated into cream and serum preparations. The physical stability test of the two preparations to evaluate their shelf life was conducted in 12 weeks of storage at room temperature, and their antioxidant activity was tested using the DPPH method. The oil has a total fatty acid content of 65% w/w with a dominant oleic acid content and the IC50 value of 147.0277 μg/mL. The Moringa seeds oil was prepared as nanoemulsion for cream and serum. The optimum formula of nanoemulsion has an average droplet size of 212 nm, a polydispersity index (PDI) of 0.355, a zeta potential of-40.6 mV, and an entrapment efficiency of 58.59%. The stability studies found the two topical preparations stable during storage, with a slightly increased in viscosity and droplet size. The antioxidant activity of cream and serum were decreased after storage for 12 weeks. © RJPT All right reserved.