Introduction: Applying research activity in hospitals in Indonesia is a kind of challenge. This study aimed to assess the research’s capacity in Prof. I.G.N.G Ngoerah General Hospital. It is related to the indicators and cultural analysis at the team, organization, and individual levels in Prof. I.G.N.G Ngoerah General Hospital. Methods: A cross-sectional study and qualitative analysis using Focus Group Discussion (FGD) were conducted in July-August 2022 at Prof. I.G.N.G Ngoerah General Hospital. The Research Capacity and Culture (RCC) validated survey measured self-reported research capacity at leadership, individual, team, and organization levels. Additionally, this study explored barriers and motivators. The rate of research capacity decisions and the domains in this study use the ROC curve. This statistical analysis used chi-square with a significance of p-value < 0.05. Results: One hundred and thirty respondents were analyzed. The individual characteristics were 45-54 years old, male, medical specialist, clinician without structural position, with a master’s degree or specialist, internal medicine department and pediatric department, 0–10 years of work history, 1–10 journal publications, and having a research history. In leadership and team capacity, most have low scores, and in individual capacity, organization and research capacity have high scores. There is a statistically strong relationship between each domain and research capacity. Conclusions: High research capacity in a hospital is very important. Evidence-based research can be used to support the services and progress of the hospital itself, especially as a teaching hospital. © 2023, Sanglah General Hospital. All rights reserved.