Introduction: assisted Reproductive Technology has transformed fertility treatment with new methods such as Intracytoplasmic Sperm Injection (ICSI), coming up with innovative ways to improve male fertility. The following paper compares the two ICSI techniques, which are mechanical and conventional, on matters of efficiency in terms of fertilization. To check for fertilization, an established morphological criterion was used. Method: this study was conducted at a fertility clinic in Bocah, Indonesia, and utilizes a cross-sectional retrospective methodology. The study analyzes four hundred and fifty-eight oocytes from twenty-four patients while focusing on days one, three, and five of fertilization to check for their success. Results: results reveal that mechanical ICSI does better than conventional ICSI in producing high-quality embryos after fertilization. Mechanical ICSI demonstrated higher precision, reduced oocyte trauma, and improved fertilization rates (68,8 % vs. 59,3 % on Day 1). The short span of this study means that the success of these methods in facilitating the carrying of long-term pregnancy is not evaluated. It is, however, valuable to note that the study found that despite the efficiency of mechanical ICSI, there are qualms about moving towards large-scale application in fertility clinics due to the high costs involved. Conclusions: this study examined conventional and mechanical ICSI in assisted reproduction including 30 individuals (mean age: 33,37 years). Mechanical ICSI yielded markedly superior-quality embryos on Days 1, 3, and 5. It enhanced embryo preservation and fertilization results, underscoring its potential as a superior strategy for improving embryo quality and fertility success. © 2025; Los autores.