Background: Mandibular asymmetry is one of the widespread conditions that affects many of the individuals. Accurate diagnosis and treatment are needed for this illness. Aim: The aim of this study is to compare mandibular asymmetry using manual, digital, and AI methods. Methods: From March to April 2025, a cross-sectional study was analysed in the Orthodontic Specialist Clinic of the Dental Hospital Faculty of Dentistry, Universitas Indonesia. This research included 15 patients who were diagnosed with mandibular asymmetry and not undergone orthodontic treatment. This study examines diagnosing asymmetry in six different facial features, including the Co-Ag, Co-Me, Ag-Me, Angle of Ag, JMSR, and AgMSR. Results: This study confirms that there was no specific difference between the measurement angle of Ag. Scientists have used a paired t-test to compare the p-value, which was greater than 0.05. The Kappa Cohen analysis was used to analyse the strength of agreement of each parameter. Conclusion: This research highlights that manual, AI and digital methods are similarly effective for measuring the angle of Ag for the treatment of mandibular asymmetry. This study may be helpful for orthodontists in treating and diagnosing mandibular asymmetry. © 2025, University of AEGEAN. All rights reserved.