Introduction: The high prevalence of Acute Respiratory Infection (ARI) in Indonesia can increase anti-infective use and affect their patterns of use. Objective: This study aimed to evaluate anti-infective use in 2015 at 3 primary health care centres, Limo Primary Health Care (LPHC), Bojongsari Primary Health Care (BPHC), and Cipayung Primary Health Care (CPHC). Material and methods: This study was descriptive analytic research using retrospective data. Samples consisted of 20,441 ARI patients' prescriptions containing anti-infective drugs during 2015. Anti-infective drugs were classified using the anatomical therapeutic chemical (ATC) classification system. Drug use was measured as Daily Defined Dose (DDD)/1000 patients/day. Drug use 90% and adherence to the National Formulary was evaluated as an indicator of the quality of prescribing drugs. Results: The highest amount of anti-infective use for ARI patients was at CPHC with a total quantity of 0.95 DDD/1000 patient/day. While at the LPHC amount of anti-infective use was 0.76 DDD/1000 patients/day and at BPHC was only 0.65 DDD/1000 patients/day. The most anti-infectives use at all primary health cares was amoxicillin. Ten anti-infectives were use at LPHC, 11 at BPHC and 7 at CPHC. Anti-infectives in the DU 90% segment at LPHC were amoxicillin, co-trimoxazole, and cefadroxil while amoxicillin was the only DU90% anti-infective used at CPHC and BPHC. The adherence to National Formulary were 70.00% at LPHC, 71.43%, at BPHC, and 63.64% at CPHC. Conclusion: The use of anti-infective varied and it showed that some did not adhere to National Formulary guidelines.