Empirical evidence suggesting that most organizations are largely inert and ultimately fail. A second perspective argues that some firms do learn and adapt to shifting environmental contexts. Changes in the environment is very dynamic and the organization is required to be able to adjust for these changes. Capabilities of the organization should be improved as a step of adaptation in order to the sustainability of organization maintained. Ambidextrous organization refers to organization which have ability to exploring potential opportunities and finding new strategies to improve. Ambidexterityconcept is important because it can improve performance and create competitive advantage of the organization. This study aims to analyze the application of the ambidexterity concept in the university regarding to the concept is generally applied to business organizations. The study used a quantitative approach and for collecting data used questionnaires, in-depth interviews, and the benchmark study as well. The results showed that the pattern of ambidexterity at the Universitas Indonesia reflects both structural and contextual ambidexterity. The perceptions of researchers in the faculties who were the respondents indicate favorable attitudes as the ambidextrous university’s indicators. © The Turkish Online Journal of Educational Technology.