Background: Enhancing orthodontic tooth movement is a crucial aspect in reducing the duration of treatment. This study aims to investigate the potential of Pangasius hypophthalmus oil in promoting tooth movement in rabbits. Materials and Methods: Forty-eight male rabbits were employed in four experimental groups (n = 12 per group): Group 1 served as the control, while Groups 2, 3, and 4 received oral doses of Pangasius hypophthalmus oil at concentrations of 250 mg/kg body weight, 500 mg/kg body weight, and 1,000 mg/kg body weight, respectively. Orthodontic appliances were placed between the lower central incisors of the rabbits, applying an orthodontic force of 100 g/mm2. The oil was administered orally to the rabbits every day for 14 days. Specimens were euthanised on day 1, 3, 7, or 14 according to the experimental group, and immunohistochemical analysis was conducted to evaluate TRAP and OPN expression in alveolar bone at compression sites. Results: The administration of Pangasius hypophthalmus oil with a dose of 1,000 mg/kg body weight led to the highest increase of orthodontic movement, indicated by a rise of TRAP and OPN expression compared to other concentrations (p < 0.05). Conclusion: The Pangasius hypophthalmus oil has the potent ability to increase the orthodontic tooth movement, indicated by the rise in TRAP and OPN expression. © 2026 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.