Introduction: More than 70% of cervical cancer cases are associated with high-risk HPV infections, especially those of type 16/18. Persistent high-risk HPV infection can cause cervical lesions and develop into cancer; therefore, early detection of HPV infection is important. Screening using HPV DNA tests, either as a single test or combined with a cervical cytological test, is recommended. This study aimed to determine the association between high-risk HPV infection and the development of cervical precancerous lesions. Methods: This cross-sectional study was conducted on 104 patients at RSUPN Dr. Cipto Mangunkusumo in 2020-2022 using secondary data from HPV DNA test results with the DiagCor GenoFlow Human Papilloma Virus Array Test and cervical cytology results from liquid-based cytology. The data were presented in tables and analyzed using SPSS version 26. Results: The prevalence of high-risk HPV infection was 30.8%, with the most common genotypes being HPV 18 & 52 (15.6%), HPV 51 (12.5%), and HPV 58 (9.4%). Cytological results from the high-risk HPV types showed that 16 out of 32 (50%) were abnormal. High-risk HPV caused 11 (78.6%) low-grade squamous intraepithelial lesions and 5 (83.3%) high-grade squamous intraepithelial lesions. It is known by the statistical analysis test that there was a significant relationship between high-risk HPV infection and cervical precancerous lesions (p=0.000). Conclusion: A significant association was observed between the occurrence of high-risk HPV infection and the development of cervical precancerous lesions. Appropriate management and supervision can be carried out in accordance with risk stratification based on screening results. Copyright (c) 2025 Indonesian Journal of Obstetrics and Gynecology. This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.