Background: Dentists are at greater risk of work-related musculoskeletal disorders (WMSDs) which can be caused by repetitive movements, improper posture and long working hours. If ergonomic principles are applied in the field of dentistry, they help to prevent occupational ergonomic health hazards and provides more comfort to the dentist and patient. Objective: To obtain information regarding dental ergonomics practice of dentists in Indonesia and its related factors. Methods: A cross-sectional study was conducted using a purposive sampling method via Google Form to 231 dentists in DKI Jakarta, Indonesia from November to December 2022. The questionnaire consisted of 34 items divided into five sections; socio-demographic, job characteristics, knowledge of dental ergonomics, attitude towards dental ergonomics and the practice of dentists regarding dental ergonomics. Bivariate analysis was conducted using Pearson Chi-square Test. Result: 76.2% dentists have poor dental ergonomics practice. There is a significant relationship (p-value < 0.05) between the practice of dental ergonomics and age group, years of practical experience and level of knowledge. Conclusion: Most Indonesian dentists (76.2%) still lack of dental ergonomics practice. Age, duration of work experience and ergonomic dental practice knowledge are significantly correlated. © 2024 The Author(s). International Journal of Dental Hygiene published by John Wiley & Sons Ltd.