Centella asiatica and Aloe vera can be useful as anti-aging agents. This research aims to evaluate the in vitro anti-collagenase, safety, and anti-aging effects of hydrogel eye masks containing Centella asiatica extract and Aloe vera extract. The anticollagenase activity was determined based on Collagenase Activity Colorimetric Assay Kit. Hydrogel eye mask containing 5% w/w C. asiatica extract and 3% w/w A. vera extract. Then a double-blind clinical trial was carried out to investigate its anti-aging effect based on collagen fiber, hydration, and skin elasticity in 30 healthy women volunteers. The hydrogel eye mask was simultaneously rubbed just beneath the right eye and the placebo rubbed beneath the left eye. The safety of the formulation was evaluated with Hen’s Egg Test–Chorioallantoic Membrane (HET-CAM) and patch test. The efficacy of the formulation was evaluated with a Skin Analyzer EH-900U. The combination of C. asiatica extract and A. vera extract showed an IC50 value was 21.912 µg/mL higher than C. asiatica and A. vera extract alone. The HET-CAM test results on the 300 mg Hydrogel eye mask showed no sign of alteration on CAM. The irritation test results indicated that the hydrogel eye mask did not cause any skin irritation and increased collagen fiber, elasticity, and moisture (p< 0.05) after 4 weeks of use. Hydrogel eye mask had good characteristics, safe and effective as skin aging cosmetics.