The pandemic period forces many changes, including achieving competencies in practical learning in the laboratory. Simulation-based education (SBE) provides learners with experiences in real patient care environments, where they can safely practice making decisions and providing care without causing harm. Virtual Reality Simulation (VRS) is a virtual simulation practice method that can increase nursing students' enthusiasm for learning. This study aims to determine undergraduate students' reaction to implementing post-appendectomy wound care practicum using Virtual Reality Simulation in the Faculty of Nursing, Universitas Indonesia. This study used a quasi-experimental design to determine the students' reactions when using VRS for the first time. A user reaction questionnaire was used after testing its validity and reliability. The questionnaire contains 24 items that consist of 11 positive questions and 13 negative questions. The sample in this study was 30 undergraduate nursing students in semesters II and IV. The results of this study indicate that the respondents were enthusiastic about doing a practicum using VRS for post-appendectomy wound care. The post-practicum questionnaire survey results evidenced this study: 53.3% of the respondents were enthusiastic, and 46.7% were not enthusiastic; the cut-off point was 91.2. The nursing students enthusiastically conducted a post-appendectomy wound care practicum using VRS. This study recommends that VRS be further developed and tested using a larger sample size. Copyright©2022 by authors, all rights reserved.