Suriname cherry (Eugenia uniflora L.) is believed to have anti-aging properties due to its main constituents, myristicin and quercetin. This study examines the anti-tyrosinase and antioxidant properties of the seed of unripe fruit extract of Suriname Cherry. The extraction process used an ultrasound-assisted extraction (UAE) method with 70% ethanol. Total phenolic and flavonoid contents were measured. Antioxidants including 2, 2-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP), 2, 2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and anti-tyrosinase activities were evaluated for the bioactivities. The antioxidant activities of E. uniflora seed extract had substantial IC50 values for DPPH 5.75 μg/mL, then FRAP values were 5197.7 μM/g. In conclusion, the Suriname Cherry seed extract has anti-tyrosinase and antioxidant properties. Furthermore, the ethanolic seed extract had anti-tyrosinase and antioxidant characteristics, suggested that the Suriname cherry plant could be a promising anti-aging agent. © 2024 The Authors. International Journal of Agriculture and Biology published by Friends Science Publishers, Faisalabad, Pakistan This is an open access article under the terms of the Creative Commons Attribution License, which permits non-commercial use, distribution and reproduction in any medium, provided the original work is properly cited