Secretome is a breakthrough for modern medicine application due to its high availability and capability to induce tissue regeneration. Research regarding cartilage recovery is vital because cartilage tissue can be found in multiple essential locations in the body but lacks regenerative properties. This research aims to identify the profile of proteins inside the secretome secreted from a dental pulp stem cell (DPSC) using HPLC and indirect ELISA, specifically, the BMP-2 protein. The mobile phase used for HPLC analysis was 2% acetonitrile solution for 30 minutes. For the indirect ELISA method, we incubated the samples overnight in a cold environment. The antibodies bound to the samples were incubated for 2 and 1 hours with a washing step after each incubation. The color was developed using TMB substrate and then analyzed in 450 nm wavelength. Our results showed that the dynamic of BMP-2 concentration follows a similar pattern to the pattern in animal cell growth. Conversely, BMP-2 concentration increased exponentially during the log phase of DPSC (6-7 days). Thus, we concluded that DPSC naturally produces high levels of BMP-2, and its concentration peaks at 6-7 days of culture. We posit that the secretome of DPSC has a promising potential as the source of signaling molecules for hard tissue engineering. © 2024 AIP Publishing LLC.