One of the most accurate methods to estimate individual’s age is tooth aspartic acid racemization analysis, of which age is estimated by inserting one’s tooth aspartic acid racemization ratio into formula gained from previous research on his/her population. Although many researches had been conducted on different population with different formula concluded, but we have not found any research conducted on Indonesian people. Our research aimed to analyze the correlation between aspartic acid racemization ratio and age of individuals from a part of Indonesian population and to conclude a formula to estimate individual’s age using aspartic acid racemization ratio. Aspartic acid amount of 14 first Premolar teeth from Javanese, North Sumatera tribe and Chinese-Indonesian people were analyzed using Ultra-high Performance Liquid Chromatography (UPLC). Aspartic acid racemization ratio of each tooth was calculated using Microsoft Excel then analyzed with SPSS 23. No relationship was shown between subjects’ age and subjects’ teeth aspartic acid racemization ratio, leading to no regression formula could be concluded to estimate subject’s age. Although we could not conclude any formula in this research, but method performed in this research was able to analyze tooth aspartic acid racemization of subjects from Javanese, North Sumatera tribe and Chinese-Indonesian people living in Jakarta, Bogor, Depok, Tangerang and Bekasi. Further exploration on subjects from specific Indonesian tribes is needed to explore the contribution of tribe difference to tooth aspartic acid racemization and to search formula to estimate individual’s age on Indonesian population © 2023,Journal of International Dental and Medical Research. All Rights Reserved.