The presence of endophytic fungi in plant species offers a promising pathway for research and innovation in natural medicinal remedies. Endophytic fungi from Saurauia cauliflora were screened for antibacterial, antioxidant, and cytotoxic activities. Two isolates, Neofusicoccum parvum P.11 and Sesquicillium intermediophialidicum P.14, exhibited significant bioactivities. Ethyl acetate extracts showed MIC values ranging from 127.7 to 256 µg/mL against multidrug-resistant bacteria, with P.14 displaying strong antioxidant activity (DPPH IC₅₀: 73.85 µg/mL). Cytotoxicity assays revealed that P.14 inhibited 74.82 % of MCF-7 breast cancer cells at 100 µg/mL, while doxorubicin (control) showed 86.94 % inhibition. GC–MS analysis identified several known bioactive compounds, suggesting their potential contribution to the observed activities. These findings highlight the pharmaceutical potential of endophytic fungi from S. cauliflora, particularly S. intermediophialidicum P.14, as a novel source of bioactive agents. Further research is required to elucidate fully their underlying mechanisms and assess their therapeutic viability, including activity-guided isolation and testing against normal cell lines. © 2025 SAAB