In this research, silver nanoparticles (AgNPs) were synthesized through a chemical reduction pathway using γ-cyclodextrin (γ-CDs) as both reducing and stabilizing agents. The synthesis was conducted under systematically varied parameters, including pH, concentrations of silver nitrate and γ-CD, and reaction time. The as-synthesized AgNPs were characterized using UV–Vis spectroscopy, Transmission Electron Microscopy (TEM), Particle Size Analyzer (PSA), and Fourier Transform Infrared (FTIR). The findings revealed that these synthesis conditions had a significant influence on the morphology of AgNPs. Alkaline pH, increased silver nitrate and γ-CDs concentrations, and prolonged reaction times promoted the formation of smaller and more uniform nanoparticles, as indicated by a blue shift in the surface plasmon resonance (SPR) peak. The optimum synthesis conditions were identified as pH 11, AgNO3 2.5 × 10−4 M, γ-CDs 0.03 M (molar ratio 1:120), and 30 min of reaction time. Under these conditions, TEM analysis showed an average particle size of 10.98 ± 1.17 nm. FTIR analysis confirmed the successful formation of AgNPs, indicated by the presence of a C ·O peak, suggesting a redox interaction between AgNO3 and γ-CDs. Zeta potential measurements demonstrated that the AgNPs exhibited values below −30 mV, indicating strong electrostatic repulsion and high colloidal stability. Furthermore, the AgNPs solution remained stable after four months of storage. This work provided valuable insights into tailoring the physicochemical properties of AgNPs for specific applications by precisely controlling synthesis parameters. © 2026 Elsevier Ltd