A microfluidic structure plays a crucial role in supporting liquid sensors. However, modeling a multilayered microfluidic structure faces challenges for permittivity sensor applications, particularly concerning the non monotonic behavior of multilayered dielectrics. These challenges arise due to the varying characteristics of the electric field (E) direction in dielectrics with low and high permittivity. The existing models exhibit significant deviations from measurement results across a wide permittivity range. To address the issue, this article proposes a quasi-static conformal approach with exponentially tapered capacitance to minimize deviations caused by nonlinear behavior. This study uses the exponential tapered permittivity ratio to adjust and modify the capacitance value. The proposed model was examined across samples with a wide range of permittivity, spanning from air of 1.0 to water of 80.0. To verify the proposed model, finite element method (FEM) simulations and experimental measurements were conducted. A three-layer configuration was prepared, i.e., glass (ϵr2 = 7.3)/liquid sample (ϵr3= 1.0-80.0)/glass (ϵr4 =7.3 ). The sample was positioned at the middle layer by using a microfluidic channel with a cuboid shape. As a result, the comparison of the quasi-static conformal approach without and with the exponentially tapered capacitance model reveals deviations in the effective permittivity (ϵr-eff) of 27.7% and 1.3%, in the characteristic impedance (Z0) of 11.1% and 0.8%, and in the total capacitance (CT) of 28.5% and 1.4%, respectively. Subsequently, the proposed sensor structure was fabricated and measured for permittivity sensor application using the resonant frequency shift approach. The measurement results, ranging from the air (ϵr3 = 1.0) to the water sample (ϵr4 =80.0), showed a frequency shift of 425.50 MHz and an average normalized sensitivity (NS) of 0.64%. This study presents a robust and accurate model, offering a practical solution for permittivity sensing. The proposed approach meets the high accuracy and sensitivity demands in diverse industrial and environmental applications. Additionally, the model is recommended for various sectors, including the biomedical industry, medicine, and material quality control. © 2001-2012 IEEE.