Burns are injuries to the skin or other organic tissue caused by exposure to extreme heat or extreme cold. Burns require a dermal substitute that acts as a matrix/scaffold which enhances epithelialization and wound healing, with favorable cosmetic and functional outcomes. This research aims to create a readily available acellular matrix scaffold using a combination of Hyaluronic Acid/Gelatin/Chitosan/Curcumin and Gelatin/Chitosan/Curcumin. The scaffold will be locally produced in Indonesia to meet the demand for burn units in hospitals across the country. The ideal result is a scaffold that is porous and is a good environment for cells to proliferate. The scaffold’s fabrication procedure comprises material preparation, combining of components and solutions, sonication, freezing, and freeze-drying or lyophilization. The characterization carried out on the prototypes are SEM, FTIR, and cell viability assay. Out of the 6 fabricated sample variations, three sample variations stood out from the others, namely Gelatin/Chitosan/Curcumin SP1 (Chitosan 10:Gelatin 30), Gelatin/Chitosan/Curcumin SP3 (Chitosan 30:Gelatin 10), and HA/Gelatin/Chitosan/Curcumin. All three show a porous structure when characterized using SEM. When characterized using FTIR, samples show the presence of peaks in hydrogen bonds, namely C-H stretching and N-H stretching and peaks in the fingerprint region which show the presence of single bonds (C-C, C-O, C-N, and C-X). Lastly, through a qualitative (direct) viability test using Gelatin/Chitosan/Curcumin SP1 sample, it was shown that cells can proliferate on the surface of the scaffold. Based on the research in this study show that scaffold that can be said to be successful is SP1 sample with a Chitosan:Gelatin ratio of 1:3 on the grounds of its sturdy and porous structure, as well as its success in cell viability tests. © The Author(s), under exclusive license to Springer Nature Switzerland AG 2025.