Lignocellulosic biorefinery is a promising route to provide valuable products to substitute utilization of fossil fuel which is unsustainable. Indonesia has abundant resources of lignocellulosic biomass. Conversion of biomass waste from agriculture, plantation, industrial processing as well as marine sources into value added products could deliver economic and environmental benefits into society. Pretreatment of lignocellulosic biomass is a crucial step in the biorefinery to break down the lignin-carbohydrate bonds, enabling utilization of cellulose, hemicellulose, and lignin. The methods may involve chemicals, psychochemical, or biological processes. The biological method is considered as an environmentally friendly process for delignification of the lignocellulosic biomass. This method commonly involves the whole cells of fungi or their secreted ligninolytic enzymes. It avoids the use of harmful solvents, release of potentially toxic byproducts, and high amounts of energy, such as when using chemical or psychochemical pretreatments. White rot fungi (WRH) are the most common organism involved in biological delignification due to its diversity and lignin degradation performance. Enzymatic delignification incorporates the cocktails of three major enzymes, i.e., laccase, lignin peroxidase, manganese peroxidase, secreted by WRF. Nevertheless, the application of biological methods often exhibits low performance due to the recalcitrant of cell wall and lignocellulose structure on fungal and enzymatic attacks. This paper will review different kinds of methods to support fungal and enzymatic delignification of biomass. This review also highlights environmentally friendly methods which operate under mild conditions operation, short time, or consume low energy such deep eutectic solvent (DES), microwave heating, and ultra-sonication methods. In addition, immobilization of lignin degrading enzymes is reported as a potential alternative to enhance the efficiency of enzymatic delignification. © 2025 Author(s).