Background: Severe air pollution has affected the health of individuals both nationally and globally. The dangerous pollutant PM2.5 could trigger oxidative damage, which has been widely studied in relation to health problems, such as lung cancer, chronic obstructive pulmonary, and cardiovascular diseases. Malondialdehyde (MDA), which is a secondary product of the lipid peroxidation of polyunsaturated fatty acids, is a marker of oxidative stress.Objective: This study aims to analyze the association between PM2.5 and MDA concentration in concrete batching plant workers as a population at risk of dust pollution.Material & Method: This research was conducted in April–May 2018 with a cross-sectional method involving 53 samples of workers chosen by simple random sampling. Beside PM2.5 and MDA concentration, other variables including smoking status, supplement consumption, age, physical activity, body mass index, years of work, and rotational shift working were also studied.Results: The results indicated a significant correlation of PM2.5 and age with MDA concentrations in concrete batching plant workers. P value between PM2.5 and MDA concentration was 0.025 with OR 15,791 CI (1,410-176,833). These findings suggest that exposure to PM2.5 leads to oxidative stress as evidenced by MDA concentration. These findings suggest that exposure to PM2.5 leads to oxidative stress as evidenced by MDA concentration. © 2019, Indian Journal of Public Health Research and Development. All rights reserved.