Morphological and molecular data reveal two distinct clades of Xanthosoma undipes (Araceae) on Mount Karang, Banten, Indonesia
Penulis:Â Windarsih, Gut;Â Hariri, Muhammad Rifqi;Â Erlinawati, Ina;Â Damayanto, I Putu Gede P.;Â Riastiwi, Indira
Informasi
JurnalJordan Journal of Biological Sciences
PenerbitHashemite University
Volume & EdisiVol. 19,Edisi 1
Halaman13 - 21
Tahun Publikasi2026
ISSN19956673
Jenis SumberScopus
Abstrak
Xanthosoma undipes, locally known in Indonesia as talas beneng, is a taro plant with edible corms. Two distinct morphological groups have been identified in three locations on Mount Karang, Banten, Indonesia. This study investigates the variation among X. undipes accessions from Mount Karang based on morphological traits and the chloroplast psbA-trnH intergenic spacer (IGS) marker. Accessions of X. undipes were collected from Mount Karang as herbarium specimens and DNA samples. Morphological data were gathered, scored, and analyzed using the UPGMA method. DNA was isolated using a modified CTAB method with 2% polyvinylpyrrolidone, leaf tissue was ground in CTAB buffer, incubated, extracted with chloroform:isoamyl alcohol (24:1), and DNA was precipitated with isopropanol, washed with 70% ethanol, and resuspended in TE. PCR amplification was performed using psbA-trnH IGS primers (forward: 5'-GTT ATG CAT GAA CGT AAT GCT C-3' and reverse: 5'-CGC GCA TGG TGG ATT CAC ATT CAC AAT-3'). DNA sequencing was conducted by 1st Base. A phylogenetic tree was constructed using the Maximum Likelihood method with 1000 bootstrap replications, using Alocasia macrorrhizos as an outgroup because it belongs to the same family but a different genus, with sequences available in NCBI. Nucleotide variations were visualized using Multalin. Morphological analysis revealed two distinct clades of X. undipes accessions from Mount Karang. Clade I consists of accessions GW1, GW3, GW12, GW14, and GW22, while Clade II consists of GW6, GW18, GW19, GW20, and GW21. Morphological characters, including corms, cormels, leaf petiole junctions, leaf sheaths, peduncles, spathes, sterile flowers, and male flowers, distinguish X. undipes accessions into two clades. Molecular analysis using the chloroplast psbA-trnH IGS marker supported the clustering observed in the morphological data. A notable difference between the two clades was found at nucleotide base position 156, where clade I had a cytosine, while clade II had a thymine. In the end, morphological and molecular evidence revealed two distinct clades among X. undipes accessions from Mount Karang. © 2026 Jordan Journal of Biological Sciences. All rights reserved.
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