Propolis is a resinous substance extracted from the plants around the honeycomb, which can be used to construct, adjust and protect the bee colony. Propolis has been known to have various benefits as traditional medicine. It contains flavonoid, caffeic acid phenethyl ester, cinnamic acid, and artepillin C, which may play a role in the immune system and promote lymphocyte proliferation. Thus, it is referred to as an immunomodulator. However, the use of liquid propolis as an active substance faces solubility problems. Propolis powder is present to overcome the limitations of the formulation of pharmaceutical preparations and can be developed as a traditional medicine with immunomodulatory activity. The purpose of this investigation was to see if propolis powder has any immunomodulatory effects using the phagocytosis macrophages method. The RAW 264.7 macrophage cell lines which had been cultured, were used in this study. Propolis samples at dosages of 100 g/ml, 50 g/ml, 25 g/ml, 12.5 g/ml, and 6.25 g/ml were used to determine the percentage of activity in RAW 264.7 macrophage cells. According to the results of this study, giving propolis powder at a dosage of 12.5 g/ml resulted in the highest macrophage capacity for phagocytosis (31±3%). In other words, propolis powder at that concentration had the greatest stimulating impact or increased phagocytosis activity the most. © 2022 American Institute of Physics Inc.. All rights reserved.