Aim: The aim of this study is to compare between ethanol and ethyl acetate rhizome extract of K.rotunda against HeLa cervical cancer cell in vitro. Material and Methods: Methods used in this research are test the chemical compound of extracts using Thin Layer Chromatography (TLC) and phytochemical screening test, also cytotoxicity test using MTT assay. Result: Ethyl acetate extract contains flavonoid, alkaloid, tannin, and triterpenoid, while ethanol extract have flavonoid, triterpenoid, and alkaloid. In addition, ethanol extract has strong cytotoxic activity (IC50 = 16,939 μg/ml) while ethyl acetate extract has moderate cytotoxic activity (IC50 = 127,9 μg/ml). Each of extracts showed significant results (p ≤ 0,05) although when compared between concentrations there are several concentrations that are not significant and also small coefficient of determinant values caused by various confounding factors. Conclusion: The ethanol extract of K.rotunda rhizome extract has the higher cytotoxicity activity compared to ethyl acetate extract of K.rotunda rhizome extract against HeLa cervical cancer cell. © 2020 Phcogj.Com.