This study presents a modified and integrated process for succinic acid production from lignocellulosic biomass wastes, incorporating pretreatment, enzymatic hydrolysis, and fermentation. Oil palm empty fruit bunches (OPEFB) and sugarcane bagasse (SB) were employed as representative biomass feedstocks. Comprehensive kinetic analysis was performed to evaluate microbial growth, product formation, and substrate consumption behaviors during fermentation. Pretreatment was conducted using a sequential combination of peracetic acid and alkaline peroxide solutions, assisted by ultrasonication, to enhance cellulose accessibility and reduce lignin content. The pretreated biomass was subsequently subjected to fermentation using two configurations: simultaneous saccharification and fermentation (SSF) for 48 h at 37 °C, and semi-simultaneous saccharification and fermentation (SSSF), consisting of a 6 h pre-hydrolysis step followed by 48 h of SSF. Kinetic analysis showed that microbial growth followed logistic behavior, succinic acid formation was well described by the modified logistic model, and substrate utilization was accurately captured by the modified Gompertz model. Variations in kinetic parameters among the biomass feedstocks highlight the influence of lignin removal efficiency and cellulose accessibility on fermentation performance. © The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2026.