Background: Plaque accumulation in teeth-supporting tissues can lead to periodontitis. Periodontitis worsens due to low Oral Health Literacy (OHL). To address this issue, a Perio-based mobile application was created. To further solidify its implementation, market research is needed to assess its long-term sustainability in Indonesia. Objective: This study analyzed the effect of age, education, and periodontal status on Perio-based mobile application usability and satisfaction. Methods: This survey-based research was conducted on 20 women aged 25-55 years who were introduced to the Perio-based mobile application. While a five commands technique did the usability test, the satisfaction test was completed through a validated questionnaire. The data were then statistically analyzed. Results: Education level significantly affects the level of Perio-based mobile application usability (p= 0.025, Correlation Coefficient:0.478). Further, the Perio-based mobile application was easy to use in the late adult group (36-45 years), undergraduate education, and periodontitis status. The highest satisfaction was seen in the late elderly group (46-55 years), high school education, and the gingivitis group. Age group and periodontal status had no significant effect on application satisfaction; however, a notable association was observed between education level and the usability of test results. Conclusion: Overall, there were no differences in the results of the application usability and user satisfaction at different ages, educational levels, and periodontal status. Perio-based mobile applications can be improved by developing an iOS version and adding autonomous features. Clinical Trial Registration Number: ISRCTN12409366 © 2025 The Author(s). Published by Bentham Open.