Gene expression profiling of vitamin D metabolism enzymes in leukemia and lymphoma patients: molecular aspect interplay of VDR, CYP2R1, and CYP24A1
Author: Anggraini Iriani, Andhika Rachman, Marsya Kaila Fatina, Rizka Kurnia Gemilang, Andi Trisnandi
Informations
JournalMolecular Biology Reports
PublisherSpringer Netherlands, Springer Science and Business Media B.V.
Volume & IssueVol. 51,Issue 1
Pages526
Publication Year2024
ISSN03014851
Source TypeGoogle Scholar
Citations
Scopus: 3
Google Scholar: 4
PubMed: 3
Abstract
BackgroundVitamin D deficiency is prevalent among the Indonesian population, particularly in individuals diagnosed with leukemia-lymphoma. The regulation of vitamin D metabolism is influenced by the expression of several enzymes, such as CYP2R1, CYP24A1, and the vitamin D receptor (VDR). This study aimed to scrutinize the gene expression profiles in both mRNA and protein levels of VDR, CYP2R1, and CYP24A1 in leukemia and lymphoma patients.MethodThe research was a cross-sectional study conducted at Cipto Mangunkusumo Hospital (RSCM) in Jakarta, Indonesia. The study included a total of 45 patients aged over 18 years old who have received a diagnosis of lymphoma or leukemia. Vitamin D status was measured by examining serum 25 (OH) D levels. The analysis of VDR, CYP2R1, and CYP24A1 mRNA expression utilized the qRT-PCR method, while protein levels were measured through …
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