Measurement of the similarity of human DNA profiles is a process which undertaken to find out a person's biological identity based on the short tandem repeat (STR) of the DNA profile. The DNA profile database was built to store DNA profile data for Indonesian citizens. The DNA profile database will assist in the identification process of individuals based on the STR value contained in the DNA profile. Fuzzy similarity measure will be used to measure the similarity of a person's STR value with DNA data stored in the database. Comparisons were made for each allele found at 15 loci of DNA profiles. Dealing with the experiments, it has shown that 98% gave the appropriate results. © 2022 IEEE.