In this research, fructose was used as a dosimeter, and ESR examined the influence of radiation dose on the dosimeter's properties. Microwave power, g-value, and decay of response tests were carried out at doses of 1, 5, and 10 kGy. The results show that fructose possesses linearity at doses of 5 × 10−2–30 kGy. The radiation detection sensitivity of fructose is better than sucrose's but less than alanine's over the entire dose range. The irradiation dose influences microwave saturation on ESR intensity, occurring at 5 and 10 kGy. The microwave power for irradiated fructose ranges from 1.00 to 2.46 mW. The ESR signal intensity increases with higher radiation doses, primarily due to the formation of more free radicals. Additionally, higher radiation doses lead to faster decay of the fructose dosimeter's response. The fructose ESR intensity stability compared to alanine and sucrose at 1, 5, and 10 kGy are alanine > sucrose > fructose, alanine > fructose > sucrose, and alanine > fructose ≈ sucrose, respectively. © 2024 Elsevier B.V.